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血管内皮细胞生长抑制因子VEGI是肿瘤坏死因子超家族新成员 ,通过诱导内皮细胞凋亡而抑制肿瘤生长。通过PCR方法将CTTHWGFTLC与VEGI2 3- 1 74氨基酸相连 ,构成嵌合分子VEGI+。原核表达的VEGI+经过纯化后 ,以非重折叠的沉淀形式进行活性实验 ,VEGI+能够抑制鸡胚尿囊膜血管新生 ,对于Lewis肺癌小鼠移植瘤 ,5mg L组抑瘤率 99.7% ,1 0mg L组抑瘤率96% ,2 5mg L组抑瘤率 83%。实验说明VEGI+通过抑制血管新生对移植瘤的早期发展起抑制作用。
Vascular endothelial cell growth inhibitor VEGI is a new member of the tumor necrosis factor superfamily, which inhibits tumor growth by inducing endothelial cell apoptosis. CTTHWGFTLC was linked to VEGI2 3- 1 74 amino acids by PCR to form the chimeric molecule VEGI +. The activity of VEGI + was purified by non-refolded sedimentation and VEGI + was able to inhibit angiogenesis in chick embryo allantoic membrane. The inhibitory rate of tumor in 5 mg L group was 99.7% and that of 10 mg L Group inhibition rate of 96%, 25mg L group tumor inhibition rate of 83%. Experiments show that VEGI + inhibits the early development of xenografts by inhibiting angiogenesis.