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对水稻脂质氧化酶(LOX)同工酶缺失的单体及聚合体人工老化实验研究表明:不同LOX的缺失突变体之间,以LOX-1,LOX-2、LOX-3全缺的D1308、D1311的发芽率始终最高,老化指数最低;LOX-1缺失所造成的影响要大于LOX-2缺失和LOX-3缺失。LOX缺失的单体及聚合体具有较好的储藏特性,LOX的缺失对保持种子生活力和延缓稻谷陈化变质具有重要的作用。进一步的分析认为LOX-1的缺失阻断或延缓了LOX-2和LOX-3所启动的脂质过氧化反应,从而延缓了稻谷的陈化变质,减轻种子生活力的下降。LOX-1可能是控制稻谷陈化变质和种子衰老的关键基因。
The results of artificial aging experiment on the degradation of rice lipoxygenase (LOX) isozymes showed that the LOX-1, LOX-2 and LOX-3 deletion of D1308 , D1311 had the highest germination rate and the lowest aging index. The effect of LOX-1 deletion was greater than that of LOX-2 deletion and LOX-3 deletion. LOX missing monomers and polymers have good storage characteristics, LOX deletion of seed to maintain viability and delay rice aging deterioration plays an important role. Further analysis showed that the deletion of LOX-1 blocked or delayed the lipid peroxidation initiated by LOX-2 and LOX-3, delayed the deterioration of rice and reduced the seed viability. LOX-1 may be the key gene controlling the rice aging and senescence.