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用焦锑酸钾沉淀的电镜细胞化学方法 ,研究了低温胁迫下枇杷幼叶细胞内 Ca2 + 水平变化。研究结果表明 ,枇杷幼叶细胞未经低温处理时 ,Ca2 +定位分布的沉淀颗粒大量出现在细胞壁、细胞间隙、质膜和液泡 ;叶绿体、细胞质和细胞核中也有一些 Ca2 +沉淀颗粒分布。枇杷幼叶经 44h、4℃低温处理后 ,在质膜和液泡膜上 Ca2 + 的沉淀增多 ,细胞质和细胞核中的Ca2 +水平增加。不抗寒品种在低温胁迫条件下可见核孔开口较大 ,有时可观察到核内容物外漏。抗寒品种在低温胁迫条件下核孔未见明显开口 ,叶绿体中类囊体不形成基粒 ,少数片层结合重叠后伸展在整个叶绿体中。而不抗寒品种类囊体则堆积形成明显的颗粒状基粒 ,类囊体片层数量较多。在 2个叶绿体之间还常可见到线粒体紧夹其间 ,但线粒体的内膜模糊不清。不抗寒品种内质网和高尔基体也较多见 ,内膜系统比较发达 ,但低温胁迫条件下膜系统易受破坏 ,膜结构模糊不清。
The changes of intracellular Ca2 + level in young loquat leaves under low temperature stress were studied by electron microscopy cytochemistry with precipitated potassium pyroantimonate. The results showed that Ca2 + localized precipitated particles appeared in the cell wall, intercellular space, plasma membrane and vacuoles when the young loquat leaves were untreated. There were also some Ca2 + precipitates in the chloroplast, cytoplasm and nucleus. After young leaves of loquat were treated with low temperature of 44 ℃ for 4h, the deposition of Ca2 + on the plasma membrane and tonoplast increased, and the level of Ca2 + in the cytoplasm and nucleus increased. Not cold-resistant varieties under low temperature stress conditions can be seen larger nuclear openings, nuclear content can sometimes be observed leakage. The cold-tolerant cultivars did not show obvious openings in the nuclear pores under low temperature stress. The thylakids of chloroplasts did not form the basal grains, and the few laminae extended and overlapped in the whole chloroplast. However, the varieties that do not resist cold weather accumulate to form granular granules, and the number of thylakoid lamellae is more. Between the two chloroplasts can often be seen between the tight mitochondria, but the inner membrane of mitochondria blurred. The endoplasmic reticulum and Golgi apparatus were also not seen in cold-resistant cultivars, and the endomembrane system was relatively developed. However, the membrane systems were easily damaged under low temperature stress and the membrane structure was unclear.