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目的通过检测福建省志贺菌的毒力基因,了解我省不同志贺菌菌群及血清型的毒力基因分布情况以及流行模式,评估不同菌型的危害性,为菌痢防控工作提供科学的实验依据。方法运用PCR扩增技术,对2005-2010年福建省腹泻病人中分离的104株志贺菌进行ipaH、set1(set1A和set1B)、sen、ial四种毒力基因的检测,分析各毒力基因的阳检率以及毒力基因的分布模式。结果 ipaH、set1(set1A和set1B)、sen、ial毒力基因的阳检率分别为100%、36.54%、66.35%、64.42%,其中set1基因在B群(福氏志贺菌)和D群(宋内志贺菌)的阳检率分别为85%和6.25%;104株志贺分为8种毒力基因分布模式命名为I-VIII,IV型是B群志贺菌的优势基因携带模式(67.5%),VI型是D群志贺菌优势基因携带模式(39.06%),同时D群中I型、VII型和V型基因模式分布也较高。结论 ipaH可作为志贺菌属的鉴定基因,set1毒力基因主要存在于福氏志贺菌(B群)中;B群志贺菌有一个集中优势的基因流行模式IV型,D群有较多的相对优势基因流行模式;说明在志贺菌进化过程中存在复杂分化形式,同时毒力基因的插入和缺失在菌型的变异和分化中有一定的相关性。
Objective To detect the virulence genes of Shigella in Fujian Province, understand the distribution and prevalence patterns of virulence genes of different Shigella strains and serotypes in Fujian Province, evaluate the harm of different strains and provide science for the prevention and control of bacillary dysentery Experimental basis. Methods PCR amplification was used to detect the virulence genes of 104 strains of Shigella isolated from diarrhea patients from 2005 to 2010 in ipaH, set1 (set1A and set1B), sen and ial, The positive detection rate and virulence gene distribution pattern. Results The positive rates of ipaH, set1 (set1A and set1B), sen and ial virulence genes were 100%, 36.54%, 66.35% and 64.42%, respectively. The positive rate of set1 gene in group B (Shigella flexneri) and group D (Shigella sonnei) positive rate was 85% and 6.25%; 104 Shiga divided into eight virulence gene distribution model named I-VIII, IV type is the B group Shigella dominant gene carrying (67.5%). Type VI was the dominant gene carrying mode of Shigella D (39.06%), and the distribution of type I, VII and V genes in group D was also higher. Conclusion ipaH can be used as the identification gene of Shigella. The virulence gene of set1 mainly exists in Shigella flexneri (group B). Shigella flexneri B group has a dominant genotype IV, D group More relative advantage of gene epidemic patterns; explain the Shigella evolutionary complex exists in the form of differentiation, while virulence gene insertion and deletion in the variation and differentiation of bacteria have a certain relevance.