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目的:观察急性呼吸窘迫综合征(ARDS)时大鼠心脏Gq/11蛋白的动态变化,从分子水平进一步阐明ARDS并发多器官功能障碍的发病机制。方法:选择雄性Wistar大鼠40只,采用尾静脉注射油酸(oleicacid,OA0.2ml/kg)法复制大鼠ARDS模型,并将其分为对照组(C组)和油酸组(OA组),又根据不同时限将其分为30、60、90、120min4个亚组,检测血气、平均动脉血压(MAP)、心功(LVSP、左室±dp/dtmax)、血浆及心肌MDA含量和LDH、CK活性;采用免疫印迹法检测心脏Gq/11蛋白含量。结果:OA组PaO2、MAP随着时间延长呈进行性降低(P<0.05～0.01),LVSP、左室±dp/dtmax逐渐降低,OA90、120min组显著低于C组(P<0.01)。OA组除30min外,血浆MDA含量、LDH活性均较C组明显升高(P<0.01),血浆CK活性从注射OA30min后开始增高(P<0.01)。OA各组心肌MDA含量、LDH活性较C组高,随时间延长呈升高趋势(P<0.05～0.01),心肌CK活性除OA30min组外均较C组高(P<0.01)。OA组除30min外,Gq/11蛋白含量随时间延长较C组分别高(24.72±24.05)%、(40.61±22.41)%、(50.84±24.49)%。形态学观察:OA30min组心肌间质轻度水肿,OA60min组有少量炎性细胞浸润,部分心肌细胞溶解、坏死,OA90、120min组出现较多炎性细胞浸润。结论:Gq/11蛋白的高表达变化可能参与了ARDS过程并发心肌损
Objective: To observe the dynamic changes of Gq / 11 protein in rat heart during acute respiratory distress syndrome (ARDS) and further elucidate the pathogenesis of ARDS complicated with multiple organ dysfunction at the molecular level. Methods: Forty male Wistar rats were randomly divided into control group (C group) and oleic acid group (OA group) by oleic acid (OA0.2ml / kg) ), And then divided into subgroups of 30, 60, 90 and 120 minutes according to different time limits. Blood gas, mean arterial pressure (MAP), cardiac function (LVSP, ± dp / dtmax), plasma and myocardial MDA content LDH, CK activity. The content of Gq / 11 protein in heart was detected by Western blotting. Results: The PaO2 and MAP in OA group decreased progressively with time (P <0.05 ~ 0.01), LVSP and ± dp / dtmax in left ventricle decreased gradually, while those in OA90 and 120min group were significantly lower than those in C group (P <0.01). In OA group, the content of MDA and the activity of LDH in plasma increased significantly (P <0.01) and the activity of CK in plasma increased from 30 min after injection of OA (P <0.01) except for 30 min. The content of MDA and the activity of LDH in OA group were higher than those in C group (P <0.05 ~ 0.01). The activity of CK in myocardium was higher than that in C group except OA30min group (P <0.01). Compared with group C, the Gq / 11 protein content in OA group was (24.72 ± 24.05)%, (40.61 ± 22.41)%, (50.84 ± 24.49)% higher than that in group C, respectively. Morphological observation: Myocardial interstitial mild edema in OA30min group, OA60min group a small amount of inflammatory cell infiltration, part of myocardial cell lysis, necrosis, OA90,120min group more inflammatory cell infiltration. Conclusion: The high expression of Gq / 11 protein may be involved in ARDS process with myocardial damage