【摘 要】
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柞蚕丝素蛋白与细胞具有特异性相互作用,作为生物医用材料具有潜在的研究和开发前景.凝胶是组织再生支架的重要材料形态之一,而迄今国内外尚无对柞蚕丝素蛋白凝胶化行为的系统性研究报道.本文研究了环境温度、pH值、柞蚕丝素溶液的浓度以及K+、Ca2+的浓度对再生柞蚕丝素蛋白的凝胶化速率的影响,研究了溶胶-凝胶转变过程中柞蚕丝素蛋白构象的变化.结果表明,再生柞蚕丝素蛋白的凝胶化时间随着溶液中丝素蛋白浓度的增大
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柞蚕丝素蛋白与细胞具有特异性相互作用,作为生物医用材料具有潜在的研究和开发前景.凝胶是组织再生支架的重要材料形态之一,而迄今国内外尚无对柞蚕丝素蛋白凝胶化行为的系统性研究报道.本文研究了环境温度、pH值、柞蚕丝素溶液的浓度以及K+、Ca2+的浓度对再生柞蚕丝素蛋白的凝胶化速率的影响,研究了溶胶-凝胶转变过程中柞蚕丝素蛋白构象的变化.结果表明,再生柞蚕丝素蛋白的凝胶化时间随着溶液中丝素蛋白浓度的增大和环境温度的升高而显著缩短.发现柞蚕丝素蛋白的溶胶-凝胶转变速率比同条件下的家蚕丝素蛋白快得多,且对溶剂
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