论文部分内容阅读
AIM:To investigate the characteristics of PPAR gammaligands induced apoptosis in liver cancer cells.METHODS:The effects of ligands for each of the PPARgamma ligands on DNA synthesis and cell viability wereexamined in BEL-7402 liver cancer cells.Apoptosis wascharacterized by Hochest33258 staining,DNA fragmentation,TUNEL and ELISA,and cell cycle kinetics by FACS.Modulationof apoptosis related caspases expression by PPAR gammaligands was examined by Western blot.RESULTS:PPARgamma ligands,15-deoxy-~(12,14)-prostaglandinJ2 (15d-PGJ2) and troglitazone (TGZ),suppressed DNAsynthesis of BEL-7402 cells.Both 15d-PGJ2 and TGZ inducedBEL-7402 cell death in a dose dependent manner,whichwas associated with an increase in fragmented DNA andTUNEL-positive cells.At concentrations of 10 and 30 μM,15d-PGJ_2 or troglitazone increased the proportion of cellswith G_0/G_1 phase DNA content and decreased those with Sphase DNA content.There was no significant change inthe proportion of cells with G_2/M DNA content.The activitiesof Caspases-3,-6,-7 and -9 were increased by 15d-PGJ2and TGZ treatment,while the activity of Caspase 8 had notsignificantly changed.CONCLUSION: The present results suggest the potential usefulness of PPAR gamma ligands for chemoprevention and trpatmpnt1 nf liwr ranrprc treatment of liver cancers
A investigate: investigate the characteristics of PPAR gammaligands induced apoptosis in liver cancer cells. METHODS: The effects of ligands for each of the PPARgamma ligands on DNA synthesis and cell viability wereexamined in BEL-7402 liver cancer cells. Apoptosis was characterized by Hochest 33258 staining, DNA fragmentation, TUNEL and ELISA, and cell cycle kinetics by FACS. Modulation of apoptosis related caspases expression by PPAR gammaligands was examined by Western blot.RESULTS: PPARgamma ligands, 15-deoxy- ~ (12,14) -prostaglandin J2 (15d-PGJ2) and troglitazone (TGZ), suppressed DNA synthesis of BEL-7402 cells. Both 15d-PGJ2 and TGZ inducedBEL-7402 cell death in a dose dependent manner, which was associated with an increase in fragmented DNA and TUNEL-positive cells. At concentrations of 10 and 30 μM , 15d-PGJ_2 or troglitazone increased the proportion of cells with G_0 / G_1 phase DNA content and decreased those with Sphase DNA content. There was no significant change in the proportion of cells with G_2 / M DNA con The activities of Caspases-3, -6, -7 and -9 were increased by 15d-PGJ2 and TGZ treatment, while the activity of Caspase 8 had not been changed. CONCLUSION: The present results suggest the potential usefulness of PPAR gamma ligands for chemoprevention and trpatmpnt1 nf liwr ranrprc treatment of liver cancers