The potato tuber moth (PTM), Phthorimaea operculella (Zeller), is one of the most economically significant insect pests for potato in both field and storage worldwide. To evaluate the infestation, reduction of potato yield and the control efficacy for PTM, field tests were conducted in two seasons by intercropping of potato as the host plant with maize as a non-host plant of PTM. Three intercropping patts were tested, which were 2 rows of potatoes with either 2, 3, or 4 rows of maize (abbreviated 2P:2M, 2P:3M, and 2P:4M), and the monocropped potato as the control, 2 rows of potatoes, without maize, (abbreviated 2P:0M). Results showed that the population and infestation of PTM in the 2P:3M intercropping patt was significantly lower than those in 2P:2M, 2P:4M and the monocropping patt of 2P:0M, due to the enhancement of natural enemies. Cumulative mines and tunneling in potato leaves in 2P:3M intercropping were significantly lower than those in 2P:2M and 2P:4M patts. The population of parasitoids and the parasitism rate of PTM in intercropping patt of 2P:3M were significantly higher than that in intercropping patt of 2P:2M, 2P:4M and monocropping patt of 2P:0M. We conclude that the potato intercropped with maize reduced the adult and larva populations, and reduced the damage from PTM by enhancing the number of parasitoids and the level of parasitism. The greatest population density of parasitoids and parasitism rate were in the intercropping patt of 2 rows of potatoes with 3 rows of maize. These data indicate that the host/non-host intercropping patts can be used as a biological control tactic against PTM by enhancing the density of natural enemies in the agro-ecosystems.