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以也门铁嫩茎段为材料,开展了旨在用于工厂化育苗的组织培养技术研究。研究结果表明,外植体在M S+3 m g/L BA+0.2~0.5 m g/L IAA培养基上,在腋芽萌发生长同时也启动其基部周围的分化;转入M S+2~3 m g/L BA+0.2~0.3 m g/L IAA的培养基上,每外植体能平均分化出1 mm以上的不定芽5~6个,不定芽在M S+0.5 m g/L BA+0.05 m g/L IAA培养基上进行增殖后,将其中高度大于1.2 cm的不定芽转入M S+0.05 m g/L BA+0.05 m g/L IAA+0.5%活性炭培养基上进行壮苗,再转入1/2M S+0.5m g/L IBA培养基生根,最后经练苗并移栽至网室内生长,获得供大田栽培的苗。保证适度的增殖率(约400%)、获得较粗壮的芽体、控制变异和简化培养条件是也门铁大规模组织培养育苗的技术关键。
Taking Yemen iron tender stem section as material, the tissue culture technology research aimed at industrialized seedling development was carried out. The results showed that the explants began to differentiate around the basal part of the axillary bud at the same time when the explants grew on the medium of M S +3 mg / L BA + 0.2-0.5 mg / L IAA. / L BA + 0.2 ~ 0.3 mg / L IAA, 5 ~ 6 adventitious buds were evenly differentiated per 1 mm explant, and adventitious buds were cultured in M S + 0.5 mg / L BA + 0.05 mg / L IAA medium, adventitious buds with a height greater than 1.2 cm were transferred to M S + 0.05 mg / L BA + 0.05 mg / L IAA + 0.5% S + 0.5mg / L IBA medium rooting, and finally by the seedlings and transplanted into the net room indoor growth, access to field cultivation seedlings. To ensure a moderate rate of proliferation (about 400%), to obtain relatively thick buds, controlling variation and simplifying the culture conditions are the key techniques for large-scale tissue culture and seedling development in Yemen.