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采用RT-PCR和RACE(cDNA末端快速扩增)方法克隆黄颡鱼MyoD(myogenic differentiation antigen,成肌分化抗原)基因的cDNA全长序列,对其进行生物信息学和在雌雄个体组织中的表达差异分析.结果表明,黄颡鱼MyoD基因编码区由810个碱基组成,编码269个氨基酸,其中第1～89个氨基酸为Basic区,第90～141个氨基酸为bHLH结构;黄颡鱼的MyoD基因与斑点叉尾鮰的同源性最高为93%.在雌雄成体组织中的表达分析显示,雄性个体中肌肉和胃组织中的表达量极显著高于雌性个体(p<0.01),雌性个体的肾脏和鳃组织中的表达量显著高于雄性(p<0.05).通过MyoD基因在雌雄个体中表现出的组织表达差异,认为这可能是造成黄颡鱼雌雄生长差异的重要因素之一.
The full-length cDNA of myogenic differentiation antigen (MyoD) was cloned by RT-PCR and RACE (rapid amplification of cDNA ends), and was bioinformatics and expressed in both sexes The results showed that the coding region of MyoD gene was composed of 810 bases, encoding 269 amino acids, of which the first to the 89th amino acids were the Basic region and the 90th to the 141th amino acids were the bHLH structure. The highest homology of MyoD gene to Channel catfish was 93% .The expression analysis in male and female adult tissues showed that the expression level of muscle and stomach tissues in male individuals was significantly higher than that in female individuals (p <0.01) Kidney and gill tissues were significantly higher than those in male (p <0.05) .Through the difference of tissue expression between MyoD gene in male and female, it is considered that this may be one of the important factors that cause the difference of male and female growth of yellow catfish.