葡萄皮尔斯病是由木质部限制性γ变形菌纲细菌Xylella fastidiosa subsp.fastidiosa(Xff)引起的一种葡萄上的毁灭性病害,可通过葡萄繁殖材料和玻璃翅叶蝉Homalodisca coagulata等昆虫媒介进行传播。该病主要在美洲的美国、墨西哥、阿根廷、秘鲁等国家发生流行(Davis et al.,1978)。最近,Su et al.(2013)证实该病在台湾发生,这是在亚洲地区的首次报道。目前国外对Xff的分子检测主要是基于单拷贝基因的常规PCR和实时荧光定量PCR方法 Pearles disease is a devastating disease on grapes caused by Xylella fastidiosa subsp. Fastidiosa (Xff), a limiting xylose-producing bacterium belonging to the xylem that can be transmitted by insect vectors such as grapevine propagation material and Homalodisca coagulata. The disease predominantly occurs in the Americas, Mexico, Argentina, Peru and other countries (Davis et al., 1978). Recently, Su et al. (2013) confirmed that the disease occurred in Taiwan, which is the first report in Asia. At present, foreign molecular detection of Xff is mainly based on single-copy PCR and real-time PCR