阿弗他口炎(RAS)是一种常见病,但病因不明。研究发现它与维生素缺乏有关,作者为此进行调查。 材料和方法 选取70例治疗期超过1.5年的RAS患者(治疗组)和50例健康者(对照组)作研究。收集空腹静脉血(内含锂肝素),把红细胞从血浆中分离出来,然后生理盐水冲洗,三硝基甲苯X-100溶液进行溶解。计算机控制读数技术测试溶解产物的转醇酶活性和辅羧酶效应(TPP效应),若TPP效应高于15％,则说明维生素B_1缺乏。 Aphthous stomatitis (RAS) is a common disease but the cause is unknown. The study found it related to vitamin deficiency, the authors investigate this. Materials and Methods 70 patients with RAS (treatment group) and 50 healthy subjects (control group) with a treatment duration of more than 1.5 years were enrolled in this study. Fasting venous blood (containing lithium heparin) was collected, the red blood cells were separated from the plasma, and then rinsed with saline and dissolved in trinitrotoluene X-100 solution. Computer-controlled readout techniques were used to test the translocase activity and the co-carboxylase effect of the lysate (TPP effect). If the TPP effect is higher than 15%, the lack of vitamin B 1 is indicated.