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目的探讨曲古菌素A(TSA)联合5-氮基-2-脱氧胞苷(5-aza-dC)对人胃癌细胞(SGC-7901)生长及其TESTIN(TES)基因表达的影响。方法将实验分为空白对照组(不加任何药物处理)、TSA(150ng/ml)、5-aza-dC(5μmo1/L)、TSA(150ng/ml)+5-aza-dC(5μmo1/L)共4组,用MTT法测定各组药物作用对SGC-7901细胞生长的影响,用流式细胞仪分析细胞周期,AnnexinV/PI检测细胞凋亡,RT-PCR分析SGC-7901细胞TESmRNA表达。结果曲古菌素A和(或)5-aza-dC分别作用24h、48h、72h后均能抑制细胞生长,联合组较单药组抑制率显著增强,且随着药物作用时间的延长,抑制率增强更明显;流式细胞仪分析及AnnexinV/PI凋亡检测发现干预胃癌细胞48h后,联合组G0/G1期比率为(85.23±2.17)%,较单药组显著增多(P<0.05);同时诱导细胞凋亡,联合组细胞凋亡率达到(32±3.25)%,比单药组明显增加(P<0.05);TSA和(或)5-aza-dC干预胃癌细胞48h后,联合组较单药组显著增强TESmRNA的表达(P<0.05)。结论曲古菌素A联合5-aza-dC干预可抑制SGC-7901细胞生长,阻止细胞周期于G0/G1期并促进细胞凋亡,抑癌基因TESmRNA表达增多均较单药组明显。
Objective To investigate the effects of TSA combined with 5-aza-dC on the growth of human gastric cancer cell line SGC-7901 and its TESTIN (TES) gene expression. Methods The experiment was divided into blank control group (without any drug treatment), TSA (150ng / ml), 5-aza-dC ) Were measured by MTT assay. The cell cycle was analyzed by flow cytometry. The apoptosis of SGC-7901 cells was detected by flow cytometry. The expression of TES mRNA in SGC-7901 cells was detected by RT-PCR. Results Trichostatin A and / or 5-aza-dC could inhibit the cell growth 24h, 48h, 72h respectively. The inhibition rate of the combination group was significantly higher than that of the single drug group, and with the prolongation of the drug effect, Flow cytometry and Annexin V / PI apoptosis assay showed that the ratio of G0 / G1 phase in the combined group was (85.23 ± 2.17)% after 48 h intervention, which was significantly higher than that in the single drug group (P <0.05) (P <0.05). After intervention of TSA and (or) 5-aza-dC for 48 h, the rate of apoptosis in the combined group reached (32 ± 3.25)%, which was significantly higher than that in the single drug group Compared with the single drug group, the expression of TES mRNA was significantly increased (P <0.05). Conclusion Trichostatin A combined with 5-aza-dC can inhibit the growth of SGC-7901 cells, arrest the cell cycle at G0 / G1 phase and promote apoptosis. The expression of tumor suppressor gene TESmRNA was significantly higher than that of monotherapy group.