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目的研究玉簪Hosta plantaginea的抗炎活性部位及其化学成分。方法萃取法制备不同极性部位,醋酸致小鼠腹腔毛细血管通透性法、棉球致小鼠肉芽肿法筛选抗炎活性部位;硅胶柱色谱等方法对活性部位进行分离纯化,根据理化性质和波谱数据进行结构鉴定。结果醋酸乙酯部位能明显抑制小鼠腹腔毛细血管通透性增高以及棉球肉芽肿;从醋酸乙酯部位分离并鉴定了10个化合物,分别为二十二烷醇(1)、β-谷甾醇(2)、豆甾醇(3)、(25R)-2α,3β-二羟基-5α-螺旋甾烷-9(11)-烯-12-酮(4)、胡萝卜苷(5)、(25R)-2α,3β-二羟基-5α-螺旋甾烷-9(11)-烯-12-酮3-O-{O-β-D-吡喃葡萄糖基-(1→2)-O-[β-D-吡喃木糖基-(1→3)]-O-β-D-吡喃葡萄糖基-(1→4)-β-D-吡喃半乳糖苷}(6)、山柰酚3-O-(2″-O-β-D-吡喃葡萄糖基)-β-D-芸香糖苷(7)、山柰酚3-O-β-D-芸香糖苷-7-O-β-D-吡喃葡萄糖苷(8)、(25R)-2α,3β,12β-三羟基-5α-螺旋甾烷3-O-[O-α-L-吡喃鼠李糖基-(1→2)-β-D-吡喃半乳糖苷](9)、(25R)-2α,3β-二羟基-5α-螺旋甾烷3-O-[O-β-D-吡喃葡萄糖基-(1→4)-β-D-吡喃半乳糖苷](10)。结论醋酸乙酯部位为玉簪抗炎的活性部位;化合物1～10均为首次从玉簪中分离得到,其中化合物10为新的天然产物。
Objective To study the anti-inflammatory active site and its chemical composition of Hosta plantaginea. Methods Different polar sites were prepared by the extraction method, the peritoneal capillary permeability of mouse induced by acetic acid and the anti-inflammatory active site of mouse granuloma were screened by silica gel. The active site was separated and purified by silica gel column chromatography. According to the physicochemical properties And spectral data for structural identification. Results The ethyl acetate fraction obviously inhibited the increase of peritoneal capillary permeability and the granuloma of cotton ball. Ten compounds were isolated from the ethyl acetate fraction and identified as docosanol (1), β- (4), daucosterol (5), (25R) -2α, 3β-dihydroxy-5α-spirostan-9 ) -2α, 3β-dihydroxy-5α-spirostan-9 (11) -ene-12- one 3-O- {O-β-D-glucopyranosyl- (1 → 2) -O- [ β-D-xylopyranosyl- (1 → 3)] - O- β -D-glucopyranosyl- (1 → 4) -β-D-galactopyranoside} Phenol 3-O- (2 "-O-β-D-glucopyranosyl) -β-D-rutinoside (7), kaempferol 3-O-β-D-rutinoside- -D-glucopyranoside (8), (25R) -2α, 3β, 12β-trihydroxy-5α-spirostan 3-O- [O- 2 -β-D-glucopyranosyl] (9), (25R) -2α, 3β-dihydroxy-5α-spirostan 3-O- [O-β- 1 → 4) -β-D-galactopyranoside] (10) .CONCLUSION The ethyl acetate fraction is the active site of anti-inflammatory activity of Hostaphora. The compounds 1-10 were isolated from Hosta for the first time, of which compound 10 is new Natural product.