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背景:Neurturin对多巴胺能神经元、运动神经元、感觉神经元、交感神经元等多种神经元具有促进存活及保护作用的研究报道甚多,关于Neur-turin对胆碱能神经元的影响报道较少。目的:探讨神经生长因子(nervegrowthfactor,NGF)和Neurturin联合应用对体外培养的神经生长因子受体阳性神经元生长发育的影响。设计:以细胞为研究对象,重复测量,验证性研究。单位:一所大学的解剖学教研室。材料:实验于2001-09/2002-06在广州医学院人体解剖教研室完成。新生的SD大鼠,培养液采用DMEM/F12培养基,另加20g/L无血清添加剂,抽滤灭菌后备用。方法:实验分4组:NGF组,NTN组,NGF+NTN组及对照组。前3组分别加入NGF,NTN,NGF+NTN,使其各浓度达到100μg/L。对照组加培养液替代。神经元原代培养新生大鼠基底前脑胆碱能神经元,然后用免疫组化结合图像分析技术观察Neurturin,NGF对培养的新生大鼠基底前脑胆碱能神经元生长发育的作用。主要观察指标:培养4,8d时新生大鼠前脑的NGFR阳性神经元存活情况和生长情况。结果:培养4d时,NTN组的各项指标均与对照组有显著性差异(P<0.05),NGF+NTN组各项指标均优于NGF组或NTN组(P<0.05);培养8d时,NTN组除突起数目外其他各项指标均与对照组无显著性差异(P>0.05),NGF+NTN组各项指标仍然优于NGF组或NTN?
BACKGROUND: Neurturin has reported a great deal of research on the survival and protection of dopaminergic neurons, motor neurons, sensory neurons, sympathetic neurons and other neurons. The effect of Neur-turin on cholinergic neurons has been reported less. Objective: To investigate the effects of nerve growth factor (NGF) and Neurturin on the growth and development of nerve growth factor receptor positive neurons cultured in vitro. Design: Cell research, repeated measurements, confirmatory studies. Unit: An anatomy department of a university. MATERIALS: Experiments were performed at Department of Human Anatomy, Guangzhou Medical College from September 2001 to June 2002. Newborn SD rats, culture medium using DMEM / F12 medium, plus 20g / L serum-free additives, filtered and sterilized after use. Methods: The experiment was divided into 4 groups: NGF group, NTN group, NGF + NTN group and control group. The first three groups were added NGF, NTN, NGF + NTN, so that the concentration of 100μg / L. Control group plus medium instead. Neurons primary cultured cholinergic neurons in basal forebrain of neonatal rats, then observed the effect of Neurturin and NGF on the growth and development of basal forebrain cholinergic neurons in cultured neonatal rats by immunohistochemistry and image analysis. MAIN OUTCOME MEASURES: Survival and growth of NGFR positive neurons in neonatal rat forebrain at 4 and 8 days after culture. Results: After 4 days of culture, the indexes of NTN group were significantly different from those of control group (P <0.05). The indexes of NGF + NTN group were better than those of NGF group or NTN group (P <0.05) , NTN group except for the number of protrusions and other indicators were no significant difference with the control group (P> 0.05), NGF + NTN group indicators are still better than the NGF group or NTN?