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采用毛细管电泳电化学检测法(CE-ED)同时测定了大豆制品中的大豆甙元和染料木素。考察了电极电位、运行缓冲液的酸度和浓度、电泳电压及进样时间对分离和检测的影响。在优化的测定条件下,上述二种组分在20min内达到基线分离。大豆甙元和染料木素在约三个数量级范围内呈线性关系,检测下限(S/N=3)为1.0×10-7g/mL,该方法已经成功的用于实际样品中大豆异黄酮的测定。
Simultaneous determination of daidzein and genistein in soy products was carried out by capillary electrophoresis with electrochemical detection (CE-ED). The effects of electrode potential, acidity and concentration of running buffer, electrophoresis voltage and injection time on separation and detection were investigated. Under the optimal conditions, the above two components reached baseline separation within 20 min. Daidzein and genistein were linear over a range of about three orders of magnitude with a detection limit (S / N = 3) of 1.0 × 10-7g / mL. This method has been successfully applied to the determination of soy isoflavones Determination.