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目的:建立高效液相色谱(HPLC)测定参松养心胶囊中人参皂苷Rb1含量的方法,为参松养心胶囊的质量标准控制提供理论依据。方法:样品经甲醇回流提取2 h,水饱和正丁醇萃取,氨试液洗涤。采用Agilent 1200RRLC高分离度快速液相色谱仪,waters XTERRAMS C18(4.6 mm×250 mm,5μm)色谱柱,柱温室温,流动相乙腈-水(53∶47),流速1.0 mL.min-1,检测波长203 nm,进样体积40μL。结果:人参皂苷Rb1进样量在5.0～200.0 mg.L-1与峰面积呈良好线性关系(r=0.998 9);平均回收率为100.31%,RSD 1.98%。结论:实验所建立的方法专属性强,准确度高,重复性好,可作为参松养心胶囊质量控制。
Objective: To establish a HPLC method for the determination of ginsenoside Rb1 in Shensong Yangxin Capsule, which provides a theoretical basis for the quality control of Shensong Yangxin Capsule. Methods: Samples were extracted by methanol reflux for 2 h, water-saturated n-butanol was extracted, and ammonia solution was washed. The mobile phase consisted of acetonitrile-water (53:47) at a flow rate of 1.0 mL · min-1 using a Agilent 1200 RLC high performance liquid chromatography with Waters XTERRA® MS C18 (4.6 mm × 250 mm, 5 μm) 1, detection wavelength 203 nm, injection volume 40μL. Results: The injection volume of ginsenoside Rb1 was in the range of 5.0 ~ 200.0 mg.L-1 and the peak area was linear (r = 0.998 9). The average recovery was 100.31% and the RSD was 1.98%. Conclusion: The method established by the experiment is specific, accurate and repeatable, and can be used as quality control of Shensong Yangxin Capsule.