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氧氟沙星（ＯＦ）是第三代喹诺酮类药物中效果最好的广谱抗菌素之一。由于它在ｐＨ２～１１范围内难溶于水与醇，给用ＨＰＬＣ法检测其含量带来一定困难。本文报告了用毛细管区带电泳（ＣＺＦ）分离检测该药的方法。由于使用ｐＨ调制样品引入技术，克服了其溶解性不好给ＨＰＬＣ法所带来的困难。其实验条件为：５０ｍｍｏｌ／Ｌ硼砂（ｐＨ１．５）为电泳缓冲液，２８０ｎｍ为紫外检测波长，２５ｋＶ为电泳电压。样品由高差进样方式引入分离毛细管（５．１２ｃｍ×５０μｍｉ．ｄ．，有效分离长度为３８．５ｃｍ）。用磺胺作内标，ＯＦ在４８～５３０ｍｇ／Ｌ及５８０～９７０ｍｇ／Ｌ范围内可进行定量分析。保留时间（ｔｒ）及相对紫外吸收（Ａ（样品）／Ａ（内标））的日内－ＲＳＤ值分别小于１．０％和２．４％（ｎ＝６）。
Ofloxacin (OF) is one of the most effective broad-spectrum antibiotics in quinolones of the third generation. Because it is difficult to dissolve in water and alcohol within the range of pH2 ~ 11, it is difficult to detect its content by HPLC. This paper reports a method for the separation and detection of this drug by capillary zone electrophoresis (CZF). Due to the use of pH-modulated sample introduction technology, it overcomes the difficulties of HPLC method due to its poor solubility. The experimental conditions: 50mmol / L borax (pH1.5) for the electrophoresis buffer, 280nm UV detection wavelength, 25kV for the electrophoresis voltage. The sample was introduced into the separation capillary (5.12cm × 50μmi.d., The effective separation length of 38.5cm) by means of height difference injection. Sulfanilamide as an internal standard, OF quantitative analysis in the range of 48 ~ 530mg / L and 580 ~ 970mg / L. Intra-day-RSD values for retention time (tr) and relative ultraviolet absorption (A (sample) / A (internal standard)) were less than 1.0% and 2.4%, respectively.