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比较了胀果甘草(Glycyrrhiza inflata)悬浮细胞在逐级放大摇瓶中的生长、黄酮产量以及营养消耗过程,以便了解其放大规律。结果表明,在250和500mL摇瓶中,细胞的最大生物量、黄酮产量以及最大比生长速率没有显著性差异,但是在1L的摇瓶中,这三种参数都显著地降低,分别比250mL摇瓶中降低了27%,30%和27%。在逐级放大的摇瓶中,氮、磷、铵浓度都随着培养时间延长而逐渐降低,尽管在1L的摇瓶中磷消耗得最慢,但三种摇瓶中磷在细胞生长对数期基本都被消耗尽了。此外,硝态氮在第18天时基本被消耗完,而铵态氮在细胞收获时仍能维持在100mg/L。因此在反应器中培养时,主要的培养条件还需进一步优化。
The growth, flavonoid yield and nutrient consumption of suspended cells of Glycyrrhiza inflata were compared in order to understand their amplification. The results showed that there was no significant difference in maximum biomass, flavonoid yield, and maximum specific growth rate in 250 and 500 mL shake flasks, but all three parameters were significantly reduced in 1 L shake flasks, respectively, over 250 mL shake Bottles dropped by 27%, 30% and 27%. The nitrogen, phosphorus and ammonium concentrations gradually decreased with increasing culture time in the graduated flasks. Although the phosphorus was the slowest in 1L shake flasks, The basic period has been exhausted. In addition, nitrate nitrogen was essentially depleted on day 18 while ammonium nitrogen remained at 100 mg / L at cell harvest. Therefore, in the reactor culture, the main culture conditions need to be further optimized.