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作者研究特异性阻断DNA甲基化的5-氮杂胞苷(5-AzaC)对从2例急性淋巴细胞白血病患者和1例慢性髓细胞白血病淋巴细胞急性变患者采集培养的细胞株NALM-18的作用。结果发现:10~(-5)或10~(-6)M5-AzaC对末端核苷酸转移酶(TdT)阳性的NALM-18株细胞的增殖有明显抑制作用,TdT活性受到显著抑制,但对DNA多聚酶α活性抑制较轻,TdT阳性细胞和花生凝集素阳性细胞显著减少;而细胞形态,E玫瑰花阳性细胞,膜表面免疫球蛋白阳性细胞及细胞浆内μ链诱导等都无变化。作者还发现5-AzaC对已去氧助
The authors studied 5-NAzaC, a specific blockade of DNA methylation, in a cell line NALM- collected from two patients with acute lymphoblastic leukemia and one patient with chronic lymphocytic leukemia acute lymphoblastic leukemia. The role of 18. The results showed that 10~(-5) or 10~(6)M5-AzaC significantly inhibited the proliferation of NALM-18 cells with terminal nucleotidyl transferase (TdT) positive, and TdT activity was significantly inhibited, but The inhibition of DNA polymerase alpha activity was lighter, TdT-positive cells and peanut agglutinin-positive cells were significantly reduced, while cell morphology, E rose-positive cells, immunoglobulin-positive cells on the surface of the membrane, and induction of mu chains in the cytoplasm did not change. The author also found that 5-AzaC has