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目的观察梓醇对L-Glu和Aβ25-35损伤PC12细胞的保护作用的异同。方法常规培养神经元样PC12细胞,预先加入梓醇及对照生理盐水24 h后分别加L-Glu 5 mmol/L和Aβ25-3520μmol/L损伤24 h,K252a干预组细胞在梓醇前1 h加入200 nmol/LK252a。MTT法测定细胞存活率,放射配基结合分析测定M2受体密度。结果梓醇100μmol/L明显提高细胞存活率(P<0.01),10μmol/L、100μmol/L升高M2受体密度(P<0.05,P<0.01),K252a部分阻断梓醇对Aβ25-35损伤细胞的保护作用,对梓醇保护L-Glu损伤的阻断作用更强。结论梓醇对L-Glu和Aβ25-35损伤PC12细胞保护作用有差异。
Objective To observe the similarities and differences of the protective effects of sterols on PC12 cells injured by L-Glu and Aβ25-35. Methods Neuron-like PC12 cells were routinely cultured. After adding hinokitiol and normal saline for 24 h, L-Glu 5 mmol/L and Aβ25-3520 μmol/L were added for 24 h. K252a cells were added 1 h before sterols. 200 nmol/LK252a. The cell viability was measured by MTT assay and the M2 receptor density was measured by radioligand binding assay. Results Sterol alcohol 100μmol/L significantly increased cell viability (P<0.01), 10μmol/L, 100μmol/L increased M2 receptor density (P<0.05, P<0.01). K252a partially blocked sterol against Aβ25-35 The protective effect of damaged cells is more potent on the protection of L-Glu from sterols. Conclusion Sterols have different protective effects on L-Glu and Aβ25-35 PC12 cells.