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本研究探讨正常人和不同疾病状态急性白血病(AL)患者Id4基因启动子区甲基化状态差异。采用甲基化特异性聚合酶链反应(MS-PCR)对正常人和AL患者骨髓进行Id4基因启动子区甲基化状况检测。结果表明:Id4基因在正常骨髓中呈完全性非甲基化状态,初治AML和ALL患者中Id4基因甲基化比例分别为84%和86%。8例复发难治的AL患者中Id4基因全部甲基化,14例Id4基因甲基化的完全缓解ALL患者中有8例在12个月内复发,而9例Id4基因非甲基化的完全缓解ALL患者中12月内复发仅1例;Id4基因呈甲基化状态的完全缓解的AML患者中12个月内复发率为62.5%,而在非甲基化的患者中12个月内复发率仅为10%,两者差异有显著性意义。完全缓解的ALL患者甲基化比例为64.3%,而完全缓解的AML患者甲基化比例为28.6%,两者差异有显著性意义。39例初治AL中,有33例Id4基因呈甲基化状态,8例复发难治的AL患者的Id4基因均呈甲基化状态,58例完全缓解的AL中,有24例Id4基因呈甲基化状态。结论:与正常人不同,AL患者中Id4基因都发生了不同程度的甲基化改变,缓解状态患者的甲基化比例低于非缓解状态患者,Id4基因甲基化模式的改变与AL的发生密切相关。
This study was to investigate the methylation status of Id4 gene promoter in normal and acute leukemia patients with different disease states. Methylation-specific polymerase chain reaction (MS-PCR) was used to detect the methylation status of Id4 gene in bone marrow of normal and AL patients. The results showed that the Id4 gene was completely unmethylated in normal bone marrow and the methylation of Id4 gene was 84% and 86% in the newly diagnosed AML and ALL patients, respectively. Id8 gene was methylated in 8 patients with relapsed and refractory AL, 8 of 14 patients with complete methylation of Id4 gene recurred within 12 months, whereas 9 patients with Id4 unmethylated Alleviating the relapse in ALL patients was only 1 case in 12 months. The recurrence rate of AML patients with Id4 gene whose methylation status was completely relieved was 62.5% in 12 months but not in 12 months in non-methylated patients The rate is only 10%, the difference between the two has a significant meaning. In patients with complete remission, the percentage of methylation was 64.3% in ALL patients and 28.6% in patients with complete remission. The difference was statistically significant. Among 39 AL patients, Id4 gene was methylated in 33 cases and methylated Id4 gene in 8 AL patients refractory to relapse. Among the 58 AL patients with complete remission, 24 cases showed Id4 gene Methylation status. CONCLUSIONS: In contrast to normal subjects, there is a different degree of methylation of Id4 gene in patients with AL, and the methylation status of patients with remission is lower than that of non-remission patients. The methylation pattern of Id4 and the occurrence of AL closely related.