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小麦抗叶锈基因Lr19来源于长穗偃麦草,表现优良的抗叶锈性,国内外发现对Lr19有毒性的小麦叶锈菌菌株的报道较少。本研究以TcLr19和Thatcher亲本以及TcLr19×ThatcherF2代单株构建的分离群体为材料,建立了与Lr19共分离的稳定的SCAR分子标记,命名为Y19SCAR982。对49个小麦抗叶锈近等基因系材料的稳定性检测结果表明,其重复性好且为Lr19特异的分子标记。对120个小麦品种检测的结果表明,该标记可有效应用于小麦抗叶锈分子辅助选择育种。
The wheat leaf rust resistance gene Lr19 was derived from Eriosphaeria barbarum and showed excellent leaf rust resistance. There were few reports of wheat leaf rust fungi strains that were toxic to Lr19 both at home and abroad. In this study, we established a stable SCAR molecular marker co-segregating with Lr19, named Y19SCAR982, using the segregating population constructed from TcLr19 and Thatcher parents and TcLr19 × ThatcherF2 generation. The stability of 49 wheat isolates from the leaf rust isogenic lines indicated that they had good repeatability and Lr19-specific molecular markers. The results of testing 120 varieties of wheat showed that this marker can be effectively applied to wheat leaf rust resistance molecular assisted selection breeding.