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目的探讨川贝母复合群之间的分子生物学亲缘关系。方法从40个随机单引物里,筛选出1个合适的引物,对各样品进行RAPD扩增,同时用亲缘关系较远的浙贝母加以比对,用所得图谱分析和归纳川贝母复合群之间的亲缘关系。结果在遗传距离为0.65处,可将所有贝母分为4大类,与形态分类学结果比较吻合;野生瓦布贝母与栽培瓦布贝母的扩增图谱十分接近;瓦布贝母居于川贝母复合群之间,与川贝母、暗紫贝母和康定贝母亲缘关系更加接近;川贝母2、康定贝母4及梭沙贝母产生了等级上的变动。结论利用DNA指纹图谱探讨川贝母复合群之间分子生物学的亲缘关系,对川贝母基源植物分类提出了新的观点。
Objective To explore the relationship between molecular biology of Fritillaria cirrhosa complex. Methods From 40 random primers, one suitable primer was screened, and each sample was amplified by RAPD. At the same time, Fritillaria cirrhosa was compared with Fritillaria cirrhosa. The relationship between. Results At the genetic distance of 0.65, all Fritillaria thunbergii were divided into four categories, which were in good agreement with the morphological classification results. The amplifications of wild Fritillaria ussuriensis Fritillaria cirrhosa complex between, and Fritillaria cirrhosa, Fritillaria and Kangdingbeimu closer relationship; Fritillaria cirrhosa 2, Kangding Fritillaria 4 and Fritillaria Fritillaria produced a level of changes. Conclusion DNA fingerprinting was used to explore the molecular biology of Fritillaria cirrhosa. The phylogenetic relationships of the Fritillaria cirrhosae were also discussed.