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①目的观察深低温保存后兔角膜内皮细胞的活性。②方法将深低温保存兔角膜与新鲜兔角膜进行内皮细胞体外培养对比观察。③结果在培养７ｄ内，深低温保存的角膜内皮细胞较新鲜角膜内皮细胞滞后１～２ｄ生长，１４ｄ以后二者逐渐趋于一致。④结论经深低温长期保存的兔角膜内皮细胞仍保持良好的生长活性
①Objective To observe the activity of rabbit corneal endothelial cells after cryopreservation. ② Methods Cryopreservation of rabbit cornea and fresh rabbit cornea endothelial cells compared in vitro culture. (3) Results During the 7 days of culture, corneal endothelial cells preserved in deep hypothermia lasted 1 ~ 2 days later than fresh corneal endothelial cells, and gradually became consistent after 14 days. ④ Conclusion The rabbit corneal endothelial cells preserved in deep hypothermia maintained good growth activity