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用10个线粒体基因为探针,对NCa不育系、保持系和可育F1的苗期叶片、幼蕾及未成熟种子的线粒体RNA进行了Northern分析。结果表明,这10个线粒体基因除atp6外,其余9个基因在同一材料的不同组织中没有表达差异,都属于组成型表达的线粒体基因。其中,orf139、orf222、atp1、cox1、cox2、cob、rrn5S、rrn26S等8个线粒体基因在不育系、保持系和可育F1的苗期叶片、幼蕾及未成熟种子中有着相同的表达,属于表达不受核基因型影响,没有组织特异性的类型;atp9基因分别在同一材料的不同组织中的转录也基本没有变化,但是在3个不同的材料间具有表达差异;可能属于表达受核基因型影响、没有组织特异性的线粒体基因。atp6基因也在3个材料的叶、蕾和种子中都产生相同大小的转录本,但是在各个材料的不同组织中存在着信号强度的差异,可能是属于表达既受核基因型影响、又有组织特异性的线粒体基因。Orf-222和orf139分别在不育系和可育F1幼蕾中产生相同大小和丰度的转录本,但是在保持系幼蕾中没有检测到转录本;orf222检测到的3条转录本分别为1.1kb、0.9kb、0.6kb,而orf139检测到0.8kb和0.6kb两条带。atp9探针在不育系和保持系幼蕾中都检测到1条0.6kb的转录本,而在可育F1幼蕾中检测到0.6kb和1.2kb的转录本。讨论了orf222、orf139、atp9基因的表达与NCa细胞质雄性不育的可能关系。
Ten mitochondrial genes were used as probes to analyze the mitochondrial RNA of NCa sterile line, maintainer line and fertile F1 seedling leaves, young buds and immature seeds. The results showed that the ten mitochondrial genes except atp6, the other nine genes in the same material in different tissues showed no difference, all belong to constitutively expressed mitochondrial genes. Among them, eight mitochondrial genes such as orf139, orf222, atp1, cox1, cox2, cob, rrn5S and rrn26S have the same expression in the seedling leaves, young buds and immature seeds of sterile F1, maintainer line and fertile F1, Belong to the expression is not affected by the nuclear genotype, there is no tissue-specific types; atp9 gene respectively in the same material in different tissues also showed little change in the transcription, but in three different materials with expression differences; may belong to the nuclear Genotype affected, there is no tissue-specific mitochondrial genes. The atp6 gene also produced transcripts of the same size in leaves, buds, and seeds of three materials, but differences in signal intensity existed in different tissues of the various materials, possibly due to the influence of the nuclear genotype Tissue-specific mitochondrial genes. Orf-222 and orf139 produced transcripts of the same size and abundance in sterile bulbs and fertile F1 young buds, respectively, but no transcripts were detected in young buds of the maintainer line. The three transcripts detected by orf222 were 1.1 kb, 0.9 kb, 0.6 kb, while orf139 detected two bands of 0.8 kb and 0.6 kb. The atp9 probe detected a 0.6 kb transcript in the male sterile line and the maintainer line, whereas the 0.6 kb and 1.2 kb transcripts were detected in fertile F1 young shoots. The possible relationship between orf222, orf139, atp9 gene expression and NCa cytoplasmic male sterility was discussed.