论文部分内容阅读
通过对露地及温室环境下有机和常规蔬菜栽培土壤采样,测定分析了5种参与土壤碳氮循环的酶活性,及其与土壤相关理化性质之间的关系。结果显示:温室及露地土壤EC值在有机生产中相应低于常规生产12%和16%;有机生产土壤微生物碳氮含量显著高于常规生产;不同生产模式下土壤酶活性差异显著,有机生产土壤中的蛋白酶、脲酶、脱氢酶、β-葡糖苷酶活性高于常规生产,而硝酸还原酶活性较常规生产低;有机与常规栽培对蛋白酶活性影响极显著(P=0.006 8),对脲酶活性影响程度达显著水平(P=0.012 4)。除脱氢酶以外,不同栽培模式环境对土壤中另外4种酶活性均有显著影响,温室栽培环境中的蛋白酶、脲酶和硝酸还原酶活性高于露地。除硝酸还原酶外,其他4种酶活性与可溶性全氮、微生物碳、微生物氮相关系数达到显著水平。分析表明,土壤酶活性受到栽培方式以及环境的影响,并且有机生产能够提高参与土壤碳氮循环的酶活性。土壤蛋白酶、脲酶、脱氢酶和β-葡糖苷酶活性能够作为表征土壤碳氮循环以及微生物活性的指标。
Through the sampling of cultivated soils of organic and conventional vegetables in the open field and the greenhouse, the enzyme activities of five kinds of soil carbon and nitrogen cycling and their relationship with soil physical and chemical properties were analyzed. The results showed that the EC of greenhouse and open-field soils were 12% and 16% less than that of conventional production in organic production, while the content of soil microbial biomass carbon and nitrogen in organic production was significantly higher than that of conventional production. The soil enzyme activities differed significantly under different production modes. The activity of protease, urease, dehydrogenase and β-glucosidase was higher than that of conventional production, while the activity of nitrate reductase was lower than that of conventional production. The effect of organic and conventional cultivation on protease activity was significant (P = 0.006 8) The degree of activity was significant (P = 0.012 4). In addition to dehydrogenase, different cultivation modes had significant effects on the activities of the other four enzymes in soil. The activity of protease, urease and nitrate reductase in greenhouse cultivation environment was higher than that in open field. In addition to nitrate reductase, the other four enzyme activity and soluble total nitrogen, microbial carbon, microbial nitrogen correlation coefficient reached a significant level. The analysis showed that soil enzyme activity was affected by the cultivation methods and the environment, and organic production could increase the enzyme activity involved in soil carbon and nitrogen cycling. Soil protease, urease, dehydrogenase and β-glucosidase activities can be used as indicators of soil carbon cycling and microbial activity.