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[Objective]To develop a reliable HPLC method for determining the dynamic change of taxol content in the root of Taxus media at different ages.[Method]The HPLC conditions were as follows:Phenomenex Luna C18 column(250 mm×4.6 mm,5 μm),mobile phase of methanol-acetonitrile-water,flow rate of 1.0 ml/min,detection wavelength of 227 nm,column temperature of 40 ℃ and injecting amount of 10 μl.[Result]The taxol was well separated under the above conditions.The linear relationship was good in the range of 1.00-32.00 mg/L(r=0.999 9),the average recovery rate and RSD values were 100.3% and 1.6% respectively.The content of taxol in the root of Taxus media at different ages was varied.[Conclusion]A reliable and convenient method was firstly established to determine the taxol content in the root of Taxus media.
[Method] The HPLC conditions were determined as follows: Phenomenex Luna C18 column (250 mm × 4.6 mm, 5 μm ), mobile phase of methanol-acetonitrile-water, flow rate of 1.0 ml / min, detection wavelength of 227 nm, column temperature of 40 ° C. and injection amount of 10 μl. [Result] The taxol was well separated under the above conditions. The linear relationship was good in the range of 1.00-32.00 mg / L (r = 0.999 9), the average recovery rate and RSD values were 100.3% and 1.6% respectively. The content of taxol in the root of Taxus media at different ages was varied. [Conclusion] A reliable and convenient method was established to determine the taxol content in the root of Taxus media.