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应用抗巨噬细胞表面分子Mac-1单克隆抗体（M1／70和M18／2）处理巨噬细胞，观察M1／70和M18／2对杜氏利曼原虫前鞭毛体入侵巨噬细胞的抑制作用。结果，经上述单抗处理后巨噬细胞，对杜氏利什曼原虫的易感性明显降低，其原虫感染率和受染巨噬细胞内入侵的原虫数量减低，原虫对巨噬细胞的入侵过程及速度也减慢。M1／70和M18／2两种单抗同时应用，则对原虫侵入巨噬细胞的抑制作用更为显著，巨噬细胞受染率为13．8％，且受染巨噬细胞内入侵的原虫数量大多仅有1～2个。提示，M1／70和M18／2单克隆抗体可以通过与巨噬细胞表面Mac－1的结合，干扰巨噬细胞表面分子上与利什曼原虫相结合的连接位点，抑制利什曼原虫对巨噬细胞的入侵。
Macrophages were treated with anti-macrophage macrophages Mac-1 monoclonal antibody (M1 / 70 and M18 / 2) to observe the inhibitory effect of M1 / 70 and M18 / 2 on the invasion of M. duratinocytes into macrophages . As a result, the macrophages treated with the above-mentioned monoclonal antibodies showed a marked decrease in the susceptibility to Leishmania donovani, a decrease in the infection rate of protozoa and the reduction of the number of protozoa invaded by infected macrophages, and the invasion process of protozoa to macrophages Speed is also slowing down. The combination of M1 / 70 and M18 / 2 showed that the inhibition of protozoal invasion into macrophages was more significant, the infection rate of macrophages was 13.8%, and the protozoal invading macrophages The number of mostly only 1 to 2. It is suggested that the Ml / 70 and M18 / 2 monoclonal antibodies can inhibit the Leishmania pairs by interfering with the binding site of Leishmania on macrophage cell surface molecules by binding to Mac-1 on the surface of macrophages Invasion of macrophages.