以抗ＣＤ９５的特异性单克隆抗体（ＣＨ－１１）诱导人Ｔ细胞Ｊｕｒｋａｔ细胞系发生凋亡，Ｊｕｒｋａｔ细胞在发生凋亡过程中出现典型的ＤＮＡ梯形片段化，以荧光染料（ＰＩ）标记的流式细胞学技术对于发生凋亡的细胞进行定量测定，证实发生凋亡的细胞百分比在各个时间点上为６．１％～４３．５％之间。以电泳泳动迁移率（ＥＭＳＡ）对于发生凋亡的细胞核中转录因子ｎｕｒ７７的表达进行了研究，发现以抗ＣＤ９５单克隆抗体诱导的活化Ｔ细胞发生凋亡时，ｎｕｒ７７转录因子表达水平有显著提高。研究结果对了解细胞凋亡时的信号转导与分子生物学机制具有重要意义。 Apoptosis of Jurkat cell line was induced by anti-CD95 specific monoclonal antibody (CH-11). Jurkat cells showed typical DNA ladder fragmentation in the process of apoptosis. Fluorescent dye (PI) labeled flow Cytometry techniques quantify apoptotic cells and confirm that the percentage of apoptotic cells is between 6.1% and 43.5% at various time points. Electrophoretic mobility shift assay (EMSA) was used to investigate the expression of nur77, a nuclear transcription factor in apoptotic nuclei. The results showed that the expression level of nur77 was significantly increased when activated T cells induced by anti-CD95 monoclonal antibody . The results of this study are of great importance for understanding the signal transduction and molecular biological mechanisms of apoptosis.