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本文提出了一种快速、简便、灵敏检测痕量多胺的新方法。用对酞内酰胺苯甲酰氯同多胺进行柱前衍生反应,以反相高效液相色谱定量测定多胺含量。在4～500 pmol范围内有良好的定量线性关系,腐胺、精脒和精胺的检测限均为0.1 pmol。
This paper presents a rapid, simple and sensitive method for the detection of trace polyamines. Pre-column derivatization of phthalocyanine benzoyl chloride with polyamine was used to quantitatively determine polyamine content by reversed-phase high performance liquid chromatography. There was a good quantitative linearity in the range of 4 ~ 500 pmol. The detection limits of putrescine, spermidine and spermine were all 0.1 pmol.