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综合运用硅胶、大孔吸附树脂、制备HPLC等柱色谱法分离纯化金鸡脚Phymatopteris hastata正丁醇部位的化学成分,从金鸡脚正丁醇提取部位中分离得到1个新化合物,采用NMR和质谱等波谱学方法鉴定新化合物的结构为(-)-表阿夫儿茶精-3,5-二-O-β-D-呋喃芹菜糖苷(1)。进一步通过测定化合物对胰岛素抵抗HepG2细胞葡萄糖消耗和骨骼肌细胞L6myc细胞葡萄糖转运子4(GLUT4)转位作用来探讨其对糖代谢的影响。抗糖尿病活性筛选结果表明,化合物1及其类似化合物-柚皮苷均能剂量依赖性提高胰岛素抵抗HepG2细胞的葡萄糖消耗量,并显著促进骨骼肌细胞L6mycGLUT4的转位作用。结果表明新化合物1和柚皮苷均具有潜在的体外抗糖尿病活性。
The chemical constituents of the n-butanol fraction of Phymatopteris hastata were isolated and purified by silica gel, macroporous adsorption resin and preparative HPLC column chromatography. One new compound was isolated from the n-butanol extraction site of goldenrod. The chemical constituents were identified by NMR and mass spectrometry The structure of the new compound was identified by spectroscopic methods as (-) - epifumeisein-3,5-di-O-β-D-furan celery glycoside (1). The effect of compound on glucose metabolism in insulin-resistant HepG2 cells and the translocation of glucose transporter 4 (GLUT4) in L6myc cells were also investigated. Anti-diabetic activity screening results showed that compound 1 and its analogous compounds, naringin, can increase the glucose consumption of insulin resistance HepG2 cells in a dose-dependent manner and significantly promote the translocation of L6mycGLUT4 in skeletal muscle cells. The results show that both new compound 1 and naringin have potential anti-diabetic activity in vitro.