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Objective:To evaluate total phenolic,antioxidant and antimicrobial activities of methanolic extracts of the Wiedemannia multifida(W.multifida)and Wiedemannnia orientalis(W.orientalis).Methods:Phosmomolybdenum assay,DPPH radical scavenging activity andβ-carotent-linoleate bleaching were used to evaluate the antioxidant capacity.Results:The total phenolics were found to be(22.45±0.60)and(9.53±0.00)mg gallic acid equivalents(GAE)/g in W.multifida and W.orientalis extracts,respectively.The predominant phenolic compounds identified by HPLC-DAD in the both extracts were rutin+ellagic acid and kaempferol.Total antioxidant capacity and DPPH radical scavenging activity of W.multifida were higher than W.orientalis.Inβ-carotene-linoleic acid system,both extracts exhibited strong inhibition against linoleic acid oxidation.Antimicrobial activity was assessed by the agar diffusion method against fifteen microorganisms.Both extracts exhibited remarkable antibacterial activity.Conclusions:The present study suggests that methanolic extracts of W.multifida and W.orientalis could be a good source of antioxidant and antimicrobial agents in foods,pharmaceuticals preparations.
Objective: To evaluate total phenolic, antioxidant and antimicrobial activities of methanolic extracts of the Wiedemannia multifida (W. multifida) and Wiedemannnia orientalis (W. orientalis). Methods: Phosmomolybdenum assay, DPPH radical scavenging activity and β-carotent-linoleate bleaching were used to evaluate the antioxidant capacity. Results: The total phenolics were found to be (22.45 ± 0.60) and (9.53 ± 0.00) mg gallic acid equivalents (GAE) / g in W. multifida and W. organalis extracts, respectively.The predominant phenolic compounds identified by HPLC-DAD in the both extracts were rutin + ellagic acid and kaempferol. Total antioxidant capacity and DPPH radical scavenging activity of W. multifida were higher than W. tropicis. In beta-carotene-linoleic acid system, both extracts showed strong inhibition against linoleic acid oxidation. Antimicrobial activity was assessed by agar diffusion method against fifteen microorganisms. Both extract oroshi significantly antibacterial activity. Conclusions: The pres ent study suggests that methanolic extracts of W. multifida and W. orientalis could be a good source of antioxidant and antimicrobial agents in foods, pharmaceuticals preparations.