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应用双向电泳和质谱联用技术,对不同乳源蛋白的差异性进行了研究。根据ImageMaster 2DPlatinum图像分析软件对不同乳源酪蛋白和乳清蛋白的双向电泳(2-DE)图谱进行蛋白斑点的匹配分析,获得21个存在于水牛奶中主要分布在低丰度蛋白区的酪蛋白差异蛋白点和24个存在于水牛奶中乳清蛋白差异蛋白点。这些差异蛋白点经质谱鉴定分析,得到4个属于水牛奶酪蛋白的主要组分和2个与水牛奶中酪蛋白有较高同源性的新组分,同时获得4个属于水牛奶乳清蛋白的主要组分和3个与水牛奶中乳清蛋白有较高同源性的组分。
Two-dimensional electrophoresis and mass spectrometry were used to study the differences of different milk protein sources. According to the ImageMaster 2DPlatinum image analysis software, the protein spots were matched by two-dimensional electrophoresis (2-DE) patterns of different milk sources of casein and whey proteins to obtain 21 samples of cheese which are mainly present in low-abundance protein regions Protein differential protein spots and 24 spots of whey protein differential proteins present in buffalo milk. Four of the main components belonging to buffalo cheese casein and two new components having high homology with casein in buffalo milk were obtained by mass spectrometry identification and analysis of four spots of buffalo milk whey protein Component and three components that have high homology to whey protein in buffalo milk.