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目的通过建立幽门螺杆菌(H.pylori)感染蒙古沙鼠的动物模型,观察H.pylori及H.pylori与N-甲基-N’-硝基-N-亚甲基胍(MNNG)联用作用后胃黏膜的组织学改变,并观察了维生素C的预防作用。方法160只SPF级蒙古沙鼠随机分为5组,每组32只。A组单用H.pylori菌液灌胃;B组在接种H.pylori后4周,摄入MNNG水(20μg/mL),连续30周;C组单用MNNG水(20μg/mL),连续30周;D组在B组基础上同时摄入加维生素C的食料;E组为空白对照组。各组分别在实验后12、36、48、52周各处死8只,取胃黏膜行组织学检查,用Warthin-starry银染、PCR和快速尿素酶法检测H.pylori。结果累计至52周肠化生和异型增生的发生率A组(87.5%,62.5%)、B组(78.1%,56.3%)显著高于C组(6.2%,6.3%)、D组(15.6%,9.4%)和E组(0%,0%),P<0.01。B组的H.pylori感染率从接种后12周的100%下降到52周的66.7%。结论H.pylori感染与胃癌发生有关,维生素C在预防肠化生和异型增生的癌前病变上有一定作用。
Objective To establish an animal model of Mongolian gerbil infected with H.pylori and to observe the combination of H.pylori and H.pylori with MNNG Histological changes of gastric mucosa were observed and the preventive effect of vitamin C was observed. Methods 160 SPF Mongolian gerbils were randomly divided into 5 groups, 32 in each group. Group A was inoculated with H.pylori bacteria only. Group B received MNNG water (20μg / mL) for 4 weeks after inoculation of H.pylori for 30 weeks. Group C contained MNNG water (20μg / mL) 30 weeks. Group D was fed with vitamin C on the basis of group B. Group E was blank control group. Eight rabbits were sacrificed at 12, 36, 48 and 52 weeks after operation respectively. Histological examination of gastric mucosa was performed. Warthin-starry silver staining, PCR and rapid urease assay were used to detect H.pylori. Results The incidence of intestinal metaplasia and dysplasia in group A was significantly higher than that in group A (87.5%, 62.5%) for 52 weeks, significantly higher in group B (78.1%, 56.3%) than in group C (6.2%, 6.3% %, 9.4%) and Group E (0%, 0%), P <0.01. The infection rate of H.pylori in group B decreased from 100% at 12 weeks after inoculation to 66.7% at 52 weeks. Conclusions H. pylori infection is related to the occurrence of gastric cancer. Vitamin C may play a role in preventing precancerous lesions of intestinal metaplasia and dysplasia.