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抗高血压新药卡托普利(甲巯丙脯酸,Ⅰ)的二硫化物代谢物(Ⅱ)在血液中能与(Ⅰ)相互转化,因此测定两者的含量甚为必要。本文将(Ⅰ)和(Ⅱ)经三丁基磷还原并与N-乙基顺丁烯亚胺制成衍生物,再转化成六氟异丙酯后用气相色谱仪附Ni-63电子捕获检出器层析柱(固定性3% OV-101和担体Chromosorb W-HP100-200(?))测定含量,获得较满意的结果。血液中(1)的测定:血样、(Ⅰ)标准贮液及内标4-乙氧基甲巯丙脯酸均配成含有N-乙基顺丁烯亚胺(1∶5克分子比)的水溶液。另取空白血1ml(内含N-乙基顺丁烯亚胺5mg)与上述贮备液配成20～200ng/ml 的
The disulfide metabolite (Ⅱ) of captopril, a new antihypertensive drug (Captopril, Ⅰ), can be transformed with (Ⅰ) in the blood, so it is necessary to determine the content of both. In this paper, (I) and (II) were reduced with TBT and made with N-ethylbutenoimine derivatives, and then converted to hexafluoroisopropyl ester by gas chromatography with Ni-63 electron capture Detector column (fixed 3% OV-101 and carrier Chromosorb W-HP100-200 (?)) Determination of content, to obtain more satisfactory results. Determination of blood (1): blood samples, (I) standard stock solution and internal standard 4-ethoxymecaptopropionin are formulated with N-ethyl maleimide (1: 5 molar ratio) Aqueous solution. Another empty blood 1ml (containing N-ethyl maleimide 5mg) with the above stock solution dubbed 20 ~ 200ng / ml