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1 材料和方法1.1 材料取本院、西安西京医院等7所医院内镜检查活检,病理诊断为异型增生的石蜡标本338例.全部标本经常规连续切片、厚度5μm,HE 染色后由两名医师根据 WHO 制定的标准进行组织学诊断与分级,ABC 酶标法免疫组化染色单克隆抗体 SC3A 由本院医学实验科制备,生物素标记的马抗鼠血清及ABC 染色试剂系美国 Veetor Lab 产品.规定Ⅱ～Ⅲ度异型增生为可能癌变的阳性标准.1.2 方法 1980-09/1992-12首次诊断的异型增生244例,经
1 Materials and methods 1.1 Materials Take the hospital, Xijing Hospital, Xi’an, seven hospitals endoscopic biopsy, pathological diagnosis of dysplasia of paraffin specimens of 338 cases. All specimens were routine serial sections, the thickness of 5μm, HE stained by two physicians According to WHO standards for histological diagnosis and classification, ABC enzyme immunohistochemical monoclonal antibody SC3A prepared by the hospital medical laboratory section, biotinylated horse anti-rat serum and ABC staining reagent is the United States Veetor Lab products. Provisions of Ⅱ ~ Ⅲ dysplasia as a possible positive standard of cancer.1.2 Methods The first diagnosis of dysplasia from September 1980 to December 1992, 244 cases of