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目的观察小剂量阿斯匹林对同种异基因移植受体大鼠慢性移植肾肾病的作用并探讨可能的作用机制。方法建立大鼠肾移植慢性排斥反应模型,移植受体大鼠随机分为2组(n=10),均自术前1d开始每日以环孢素A5mg/kg灌胃,治疗组每日给予阿斯匹林5mg/kg灌胃,对照组每日给予生理盐水2ml灌胃,术后8周采静脉血测定血清肌酐、尿素氮水平;处死动物取移植肾组织进行H-E染色及免疫组化染色(测定TGF-β1表达)。结果阿斯匹林治疗组大鼠血肌酐、尿素氮升高程度,组织病理损害及TGF-β1表达程度均较生理盐水对照组轻(P<0.05)。结论肾移植术后在常规抗排斥治疗基础上使用小剂量阿斯匹林对慢性移植肾肾病有治疗作用,其机制可能与致纤维化因子TGF-β1的表达减少有关。
Objective To observe the effect of low-dose aspirin on chronic allograft nephropathy in allograft recipients and explore its possible mechanism. Methods Rat models of chronic allograft rejection were established. Rat recipients were randomly divided into 2 groups (n = 10). All rats were given intraperitoneal cyclosporine A5mg / kg once daily before operation. The rats in treatment group were given daily Aspirin 5mg / kg orally, the control group were given 2ml of saline daily intragastric administration of venous blood 8 weeks after the determination of serum creatinine, urea nitrogen levels; executed animals were transplanted kidney tissue HE staining and immunohistochemical staining (Measurement of TGF-β1 expression). Results Serum creatinine, elevated blood urea nitrogen, histopathological damage and the expression of TGF-β1 in aspirin treatment group were lower than those in normal saline control group (P <0.05). CONCLUSIONS: After low-dose aspirin treatment, renal allografts have a therapeutic effect on chronic allograft nephropathy after conventional renal transplantation. The mechanism may be related to the decrease of TGF-β1-induced fibrosis factor.