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在pH 7.4、0.1mol.L-1 Hepes条件下,由Tb3+荧光测得Tb-EHPG条件稳定常数log K为13.95±0.13;EHPG与金属离子结合后分子内O…H-O型氢键断裂而导致荧光被淬灭;HBED与金属离子结合后分子内N…H-O型氢键断裂而导致荧光增强,荧光变化均与结合的金属离子的原子量成反比.由此总结了稀土离子与HBED,EHPG结合的规律.并用荧光方法推断了脱铁伴清蛋白N-,C-端结合部位酪氨酸残基的分子内氢键类型.
Under the conditions of pH 7.4, 0.1mol.L-1 Hepes, the Tb_EHPG conditional stability constant log K was 13.95 ± 0.13 measured by Tb3 + fluorescence; the intramolecular O ... HO hydrogen bond of EHPG combined with metal ions resulted in fluorescence Is quenched, and HBED binds to metal ions, which leads to the fluorescence enhancement due to the cleavage of the hydrogen bonds of HO type HO in the molecule, and the fluorescence changes are inversely proportional to the atomic weights of the bound metal ions, thus the law of the combination of rare earth ions with HBED and EHPG The intramolecular hydrogen bonding of tyrosine residues in the N- and C-terminal binding sites of deferro-accompaniment albumin was deduced by fluorescence method.