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采集大宝山矿区重金属污染的土壤样品,采用稀释涂布平板法对土壤样品中抗铅菌株的筛选,进一步在含不同铅浓度的培养液中进行驯化;通过对该菌株的形态观察、一系列生理生化试验、以及16S r DNA序列的比对研究进行鉴定;利用原子荧光光度计测定其对发酵液中铅的吸附能力。结果表明,分离获得的抗铅菌株在铅浓度为500 mg·L-1的培养液中长势良好,鉴定为类短短芽孢杆菌(Brevibacillus parabrevis),该菌株在含铅浓度为300mg·L-1的液体培养情况下,培养20 h左右,对发酵液中铅的去除效率高,高达30.27%。
The heavy metals contaminated soil samples were collected from Dabashan Mining Area. The anti-lead strains in soil samples were screened by dilution coating method and further acclimated in culture solution containing different lead concentrations. Through the morphological observation of the strains, a series of physiological Biochemical tests, and 16S r DNA sequence alignment of the study identified; use of atomic fluorescence spectrophotometer determination of the adsorption capacity of lead in the fermentation broth. The results showed that the isolated anti-lead strains grew well in the culture medium with the lead concentration of 500 mg · L-1, and was identified as Brevibacillus parabrevis. The strain was tested at a concentration of 300 mg · L -1 In the case of liquid culture, the removal rate of lead in the fermentation broth was about 30.27% when cultured for about 20 h.