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目的:采用HPLC法测定小儿健脾颗粒中尿囊素的含量。方法:采用ZORBAX SB-C_(18)(250 mm×4.6mm,5μm)色谱柱,以乙腈-0.005 mol·L~(-1)磷酸二氢钾(0.8:100)作为流动相,流速为0.8 ml·min~(-1),柱温:30℃,检测波长:224 nm。结果:尿囊素在21.5～430.0μg·ml~(-1)范围内有良好的线性关系,平均加样回收率为97.92%,RSD=1.12%。结论:本方法快速简便,结果准确,可用于该制剂中尿囊素的定量分析。
Objective: To determine the content of allantoin in children’s Jianpi granule by HPLC. METHODS: The mobile phase was acetonitrile-0.005 mol·L -1 potassium dihydrogen phosphate (0.8: 100) with a mobile phase of ZORBAX SB-C 18 (250 mm × 4.6 mm, 5 μm) ml · min ~ (-1), column temperature: 30 ℃, detection wavelength: 224 nm. Results: Allantoin had good linearity in the range of 21.5-430.0 μg · ml -1 with an average recovery of 97.92% and RSD of 1.12%. Conclusion: The method is rapid and simple, accurate and can be used for the quantitative analysis of allantoin in this preparation.