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本文着重介绍了利用茎尖培养技术快速繁殖葡萄优良品种苗木的技术措施。试验结果表明:以MS培养基(大量元素减半)附加1.0mg/升6BA、0.01mg/升NAA、100mg/升LH、2％蔗糖、0.6％琼脂,接种长度约2mm的白羽、红玫瑰、二号白大粒、巨峰等品种的茎尖,皆可直接发育形成分生侧芽能力极强的芽丛。芽丛每个月切分继代培养一次,一个茎尖在一年内繁殖新芽数将超过100万个。适当调整激素种类和浓度或其他措施,即可依次完成成苗、生根、移栽成活等步骤,为葡萄快速育苗提供了新途径。
This article focuses on the use of shoot tip culture technology to quickly breed fine varieties of seedlings of the technical measures. The results showed that white feather and red rose of about 2 mm in length were inoculated with 1.0 mg / L 6BA, 0.01 mg / L NAA, 100 mg / L LH, 2% sucrose and 0.6% agar in MS medium On the 2nd white grain, Kyoho and other varieties of the shoot tip, can be directly developed to form a strong lateral shoot bud buds. The shoots are cut and subcultured once a month, and the number of shoots sprouting in one year will exceed 1 million. Proper adjustment of hormone types and concentrations or other measures can be followed by the completion of seedlings, rooting, transplanting survival steps, for the rapid breeding of grapes provides a new way.