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目的研究黄皮属植物黄皮Clausena lansium茎枝的化学成分。方法采用大孔吸附树脂、硅胶柱色谱、反相柱色谱、凝胶柱色谱以及制备HPLC等方法进行分离纯化,运用紫外光谱、质谱和核磁共振谱等方法鉴定化合物的结构。结果从黄皮茎枝的95%乙醇提取物的正丁醇萃取部位分离得到15个化合物,分别鉴定为1,1′,1″,1′′′,1′′′′-三十碳内五酰胺(1)、4-羟基-2,6-二甲氧基苯酚-6′-O-紫丁香酰-β-D-吡喃葡萄糖苷(2)、4-羟基-2,6-二甲氧基苯酚-6′-O-香草酰-β-D-吡喃葡萄糖苷(3)、4-羟甲基-2-甲氧基苯酚-6′-O-紫丁香酰-β-D-吡喃葡萄糖苷(4)、4-羟基-2-甲氧基苯酚-6′-O-紫丁香酰-β-D-吡喃葡萄糖苷(5)、紫丁香苷(6)、松柏苷(7)、3,4,5-三甲氧基苯酚-O-β-D-吡喃葡萄糖苷(8)、tinotuberide(9)、(E)-异松柏苷(10)、苯乙基-O-β-D-吡喃葡萄糖苷(11)、araliopsine(12)、geibalansine(13)、全缘喹诺酮(14)、γ-花椒碱(15)。结论化合物1~14为首次从该种植物中分离得到,化合物1~11为首次从该属植物中分离得到,化合物1首次从天然产物中分离得到。
Objective To study the chemical constituents of Clausena lansium stems of the genus Huangpi. Methods The compounds were isolated and purified by macroporous adsorption resin, silica gel column chromatography, reverse phase column chromatography, gel column chromatography and preparative HPLC. The structures of the compounds were identified by ultraviolet spectrum, mass spectrometry and nuclear magnetic resonance spectroscopy. Results Fifteen compounds were isolated from the n-butanol extract of 95% ethanol extract of the stems of yellow peel and identified as 1,1 ’, 1 “, 1”, 1 “” - (1), 4-hydroxy-2,6-dimethoxyphenol-6’-O-laeosyl-β-D-glucopyranoside (2) Methoxyphenol-6’-O-vanillyl-β-D-glucopyranoside (3), 4-hydroxymethyl-2-methoxyphenol-6’-O- - glucopyranoside (4), 4-hydroxy-2-methoxyphenol-6’-O-laeosyl-β-D-glucopyranoside (5), syringin (6) (7), 3,4,5-trimethoxyphenol-O-β-D-glucopyranoside (8), tinotuberide (9), β-D-glucopyranoside (11), araliopsine (12), geibalansine (13), total quinolone (14) and γ- The compounds 1-11 were isolated from this genus for the first time. Compound 1 was isolated from natural products for the first time.