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目的 :探讨康复期SARS患者外周血T细胞亚群及其相关活化分子的变化。方法 ::对出院后的SARS患者 ,采用统一调查问卷及实验室检测进行随访研究。两次随访中 ,应用流式细胞仪检测了我院 70余例经中西医结合治疗的康复期SARS患者外周血中CD3+ 、CD4 + 、CD8+ 、CD8+ CD2 8+ 、CD8+CD2 8-、CD3+ CD2 5 + 、CD3+ CD6 9+ 、CD3+ HLA DR + T细胞的百分率及CD4 + /CD8+ T细胞的比率的改变 ,并以描述性分析及t检验进行比较分析。结果 :两次随访中 ,CD3+ 、CD4 + 和CD8+ CD2 8+ T细胞的百分率 (均值 )及CD4 + /CD8+ T细胞的比率 ,均明显低于正常值 (P <0 .0 5 ) ,而CD8+CD2 8-T细胞的百分率则显著增高 (P <0 .0 1)。第 2次随访中 ,检测的CD3+ 、CD8+ 、CD3+ CD2 5 + 、CD3+ CD6 9+ 及CD3+ HLA DR + T细胞的百分率 (均值 ) ,及CD4 + /CD8+ T细胞的比率 ,与第 1次的相比较差异较显著 (P <0 .0 1) ,其余项目差异均不明显。结论 :随着康复期的延长 ,患者的免疫功能逐渐恢复 ,病毒对T细胞活化的影响逐渐减少。
Objective: To investigate the changes of T lymphocyte subsets and their related activated molecules in peripheral blood of convalescent SARS patients. Methods :: A follow-up study was conducted on patients with SARS after discharge using a uniform questionnaire and laboratory tests. In two follow-ups, the levels of CD3 +, CD4 +, CD8 +, CD8 + CD2 +, CD8 + CD2 +, CD3 + CD2 in peripheral blood of more than 70 patients with convalescent SARS treated by Integrative Chinese and Western Medicine were detected by flow cytometry 5 +, CD3 + CD6 9+, CD3 + HLA DR + T cells and the ratio of CD4 + / CD8 + T cells were compared and analyzed by descriptive analysis and t test. Results: The percentages of CD3 +, CD4 + and CD8 + CD8 + T cells and the ratio of CD4 + / CD8 + T cells in two follow-up were significantly lower than the normal values (P <0.05) + CD2 8-T cells was significantly higher (P <0.01). The percentages (mean) of CD3 +, CD8 +, CD3 + CD25 +, CD3 + CD6 9+, and CD3 + HLA DR + T cells tested at the second follow-up and the percentage of CD4 + / CD8 + The difference was significant (P <0. 01), the other items were not significantly different. CONCLUSION: With the prolongation of convalescence, the immune function of patients gradually recovered, and the effect of virus on T cell activation decreased gradually.