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目的分离并鉴定板栗种仁的化学成分。方法采用硅胶柱色谱、凝胶柱色谱、氧化铝柱色谱和重结晶等多种分离方法对板栗种仁的95%(φ)乙醇溶液回流提取物进行分离,并通过NMR、ESI-MS和TLC等多种方法对分离得到的化合物进行结构鉴定。结果分离得到6个化合物,分别鉴定为对羟基苯甲酸(p-hydroxy benzoic acid,1),原儿茶酸(protocatechuic acid,2),没食子酸(gallicacid,3),胡萝卜苷亚油酸酯(daucosterol-6′-linoleate,4),麦角甾-6,22-二烯-3β,5α,8α-三醇(ergost-6,22-diene-3β,5α,8α-triol,5),3′,3″-di methoxylarreatricin(6)。结论其中化合物4-6为首次从栗属植物中分离得到。
Objective To isolate and identify the chemical constituents of chestnut seed kernels. Methods 95% (φ) ethanol extracts of chestnut seeds were separated by silica gel column chromatography, gel column chromatography, alumina column chromatography and recrystallization and separated by NMR, ESI-MS and TLC. Various methods were used to identify the structures of the isolated compounds. As a result, six compounds were isolated and identified as p-hydroxybenzoic acid (1), protocatechuic acid (2), gallic acid (3), and carotenoid linoleate ( Daucosterol-6′-linoleate, 4), ergohexa-6,22-diene-3β,5α,8α-triol (ergost-6,22-diene-3β,5α,8α-triol,5),3′ ,3′′-di methoxylarreatricin(6).Conclusion Among them, compounds 4-6 were isolated from Castanea for the first time.