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目的 探讨慢性乙肝病毒携带者的细胞免疫功能变化与血清 HBVDNA关系。方法 应用流式细胞仪直接免疫荧光法检测 5 0例慢性乙肝病毒携带者外周血 T淋巴细胞亚群百分率 ,并以 2 0例正常健康人为对照组进行比较。结果 乙肝病毒携带者外周血 CD3+ 细胞百分率与正常对照组比较 ,无统计学差异 (P>0 .0 5 )。 Th细胞百分率较正常对照组明显降低 (P<0 .0 5 ) ;Tc细胞百分率则明显升高 (P<0 .0 1 ) ;Th/ Tc明显降低 (P<0 .0 1 )。HBVD-NA (+)组与 HBVDNA (- )组相比 ,CD3+ 细胞百分率无统计学差异 (P>0 .0 5 ) ,Th细胞百分率有降低趋势 ,Tc细胞百分率有升高趋势 ,而 Th/ Tc明显下降 (P<0 .0 1 )。结论 HBV感染可导致乙肝病毒携带者细胞免疫功能改变 ,HBVDNA复制增加可进一步导致乙肝病毒携带者细胞免疫功能紊乱
Objective To investigate the relationship between the changes of cellular immune function and serum HBVDNA in chronic hepatitis B virus carriers. Methods The percentage of T lymphocyte subsets in peripheral blood of 50 chronic hepatitis B virus carriers was detected by flow cytometry direct immunofluorescence method and compared with 20 normal healthy controls. Results The percentage of CD3 + cells in peripheral blood of patients with hepatitis B virus infection was not significantly different from that of the control group (P> 0.05). The percentage of Th cells was significantly lower than that of the normal control group (P <0.05); the percentage of Tc cells was significantly increased (P <0.01); Th / Tc was significantly decreased (P <0.01). There was no significant difference in the percentage of CD3 + cells between HBVD-NA (+) group and HBVDNA (-) group (P> 0.05), the percentage of Th cells decreased, the percentage of Tc cells increased, while Th / Tc decreased significantly (P <0.01). Conclusion HBV infection can lead to the change of cellular immune function in hepatitis B virus carriers. The increase of HBVDNA replication may further lead to the cellular immune function disorder of hepatitis B virus carriers