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蜕壳是甲壳动物常见的生长发育现象,但对调控蜕壳与生长的内在机制尚缺乏足够了解。本研究在室内条件下,对中华绒螯蟹(Eriocheir sinensis)一个蜕壳周期内的个体蜕壳与生长现象进行了连续观察,分析了2个蜕壳相关基因,即蜕皮激素受体基因(Ec R)和维甲类X受体基因(RXR),以及1个生长相关基因肌肉生长抑制素基因(MSTN)的表达及其与生长性状的相关性。结果发现,中华绒螯蟹在蜕壳后会出现一个跳跃式生长期,之后进入了一个缓慢持续上升过程,当营养物质积累到一定程度(肥满度达60%左右时)时启动下一次蜕壳;MSTN基因的表达与壳长(r=﹣0.450,P<0.05)、壳宽(r=﹣0.410,P<0.05)增长率呈显著负相关,而与肥满度呈显著正相关(r=0.450,P<0.05),Ec R和RXR基因的表达与体重、壳长和壳宽的增长率均没有显著相关性;相对来说,MSTN在蜕壳后的表达量越高,则增重率越小;而Ec R和RXR在蜕壳后表达量越高,其增重率越大。本研究结果表明,中华绒螯蟹在蜕壳后其生长具有一定的规律性,肥满度可以作为衡量中华绒螯蟹体内营养积累启动蜕壳的指标,Ec R、RXR及MSTN基因表达与生长表型具有一定的相关性。
Moulting is a common growth and development phenomenon of crustacean, but it still lacks sufficient understanding of the internal mechanisms that regulate the development of shell and growth. In this study, we observed the phenomenon of individual molt and growth during a molt cycle in Eriocheir sinensis under indoor conditions, and analyzed the correlation between two ecdysone related genes (Ec R and RXR, as well as the expression of a growth-related gene myostatin gene (MSTN) and its relationship with growth traits. The results showed that there was a leaping growth stage after the molting of Eriocheir sinensis, and then entered a slow and steady rising process. When the nutrients accumulated to a certain extent (when the fatness reached 60%), the next shedding The expression of MSTN gene was negatively correlated with the growth rate of shell length (r = -0.450, P <0.05) and shell width (r = -0.410, P <0.05) = 0.450, P <0.05). There was no significant correlation between the expression of EcR and RXR gene and the growth rate of shell length and shell width. Relatively speaking, the higher the expression level of MSTN was The smaller the rate, the higher the expression level of Ec R and RXR after exfoliation was and the higher the weight gain rate was. The results of this study showed that the growth of Eriocheir sinensis has certain regularity after molting, and the plumpness can be used as a measure of the initiation of molting in the body of Eriocheir sinensis. EcR, RXR and MSTN gene expression and growth Phenotype has some relevance.