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目的:建立高效液相色谱法测定龙胆泻肝丸中黄芩苷的含量。方法:采用Spherigel C18高效液相色谱柱(4.6mm×200mm,5μm),甲醇-水-冰醋酸(50∶50∶1)为流动相,流速1.0ml/min,检测波长276nm。结果:黄芩苷在25～200μg/ml范围内,峰面积与其浓度线性关系良好(r=0.9999),平均回收率为98.92%,RSD为0.71%(n=6)。结论:本法简便、准确、重现性好,可用于该制剂的质量控制。
Objective: To establish a HPLC method for the determination of baicalin in Longdanxiegan Pills. METHODS: A Spherigel C18 HPLC column (4.6 mm×200 mm, 5 μm) was used with methanol-water-glacial acetic acid (50:50:1) as the mobile phase. The flow rate was 1.0 ml/min. The detection wavelength was 276 nm. RESULTS: Baicalin was in the range of 25-200 μg/ml, and the linear relationship between peak area and concentration was good (r=0.9999). The average recovery was 98.92% and the RSD was 0.71% (n=6). Conclusion: This method is simple, accurate and reproducible. It can be used for the quality control of the preparation.