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染色质的组成成分,组蛋白和非组蛋白在特异的蛋白激酶作用下可以发生磷酸化修饰,组蛋白和非组蛋白的磷酸化和脱磷酸化可能在染色质的结构,基因表达以及DNA复制中起着重要的作用。本文比较是小鼠腹水型肝癌细胞核和正常小鼠肝细胞核内酸溶性蛋白质及其磷酸化的差异。正常小鼠肝细胞核酸溶性蛋白质的电泳染色图谱有一条明显可见的组蛋白H_1~0蛋白带,而对小鼠腹水型肝癌来说,此带极浅,但在腹水型肝癌细胞核酸溶性蛋白质的电泳染色图谱上可见到表观分子量约为68K的一条蛋白带,而正常小鼠肝未见此带。此外,从电泳胶片~(32)P放射自显影图谱可见腹水型肝癌组蛋白H_1,H_2A和非组蛋白带Ⅱ(MW43K),带Ⅲ(MW.67K)带Ⅳ(M.w.97K)磷酸化程度明显高于正常小鼠肝。
Chromatin components, histones and non-histones can undergo phosphorylation under the action of specific protein kinases, and histone and non-histone phosphorylation and dephosphorylation may be in chromatin structure, gene expression, and DNA replication. It plays an important role. This article compares the differences in acid-soluble proteins and their phosphorylation in the nuclei of mouse ascitic liver cancer cells and normal mouse liver cells. The electrophoretic staining pattern of nucleic acid-soluble proteins in normal mouse hepatocytes has an obvious visible histone H_1~0 protein band, whereas for mouse ascites type liver cancer, this band is extremely shallow, but in ascites-type hepatocellular carcinoma cells, nucleic acid soluble proteins A protein band with an apparent molecular weight of approximately 68K was observed on the electrophoresis staining pattern, whereas no normal liver was found in this band. In addition, histological H ~1, H_2A and non-histone bands II (MW43K) of ascites-type HCC, and phosphorylation of band III (MW97K) IV (Mw97K) were evident from the ~(32)P autoradiography of electrophoretic film. Higher than normal mouse liver.