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目的:观察氨溴索(AMB)对流感嗜血杆菌生物膜(BF)的作用以及对BF菌的杀菌作用。方法:30株流感嗜血杆菌菌株从腺样体肥大患儿术中切除的腺样体组织分离,选取具有较强成膜能力的2株细菌,96微孔板法培养细菌形成BF,结晶紫染色法测定不同浓度AMB对2株细菌BF的影响,并用扫描电镜观察细菌BF形态结构的改变;平板计数法检测AMB对BF菌杀菌作用。结果:结晶紫染色法示,当AMB浓度分别达到0.25mg/ml和0.49mg/ml时,2株细菌BF吸光度值较对照组差异有统计学意义(P<0.01),扫描电镜发现BF结构受到破坏;菌落计数显示AMB对流感嗜血杆菌BF菌有杀菌作用,且剂量依赖效果明显。结论:AMB在体外对流感嗜血杆菌BF具有破坏和清除功能,并对BF菌具有杀菌作用。
Objective: To observe the effect of ambroxol (AMB) on Haemophilus influenzae biofilm (BF) and bactericidal effect on BF bacteria. Methods: Thirty strains of Haemophilus influenzae were isolated from adenoid tissue removed from adenoid hypertrophy patients. Two strains of bacteria with stronger ability of membrane formation were selected. Bacterium was cultured in 96-well plate to form BF and crystal violet Staining method was used to determine the effect of different concentrations of AMB on the two strains of bacteria BF. The morphological changes of the bacteria were observed by scanning electron microscopy. The bactericidal effect of AMB on the bacteria was detected by plate counting. Results: Crystal violet staining showed that when the concentration of AMB reached 0.25mg / ml and 0.49mg / ml respectively, there was a significant difference (P <0.01) in BF absorbance between two strains of bacteria, and the BF structure was found by scanning electron microscopy Damage; colonies count showed AMB Haemophilus influenzae BF bactericidal effect, and the dose-dependent effect. Conclusion: AMB can destroy and eliminate Haemophilus influenzae type bF in vitro and has bactericidal effect on BF bacteria.